Wnt Signal pathway
The Wnt signaling pathways are a group of signal transduction pathways made of proteins that pass signals from outside of a cell through cell surface receptors to the inside of the cell. Wnt signaling pathways are activated by the binding of a Wnt-protein (a large family of secreted glycoproteins, such as wnt3A, Wnt1, and so on) to a family receptor. The Wnt signaling pathway plays important roles in cell cell proliferation, differentiation and survival and many other cell developing aspects.
GenTarget developed a set of reporting lentivirus for monitoring or manipulating the Wnt pathway’s activity in any of your desired cell types. Those reporting lentivirus has a luminescent report (Luciferase, Renilla Luc) or a fluorescent report (GFP, RFP) or a secreted SEAP report, under the Win-responsive promoter (that contains 8xTcf tandem repeats as the transcriptional response element). Once the inducer (like Wnt3A protein) is present and react to Tcf motif, the luminescent or fluorescent report or SEAP reporter is expressed whose signal can be easily readout via luciferase assay or by fluorescent microscope, or SEAP reporter assay.
Those reporting lentivirus also express a fluorescent selection marker or an antibiotic selection marker under a constitutive RSV promoter, which makes it easier to select the stably infected Wnt signal reporting cells (like generate WNT sensor cell lines), or this constitutively expressed fluorescent marker (when applicable) can be serve as the internal normalization control. See the scheme below for lentivector’s core expression cassette. Click the Product Manual (pdf) for all available Wnt signal products.
