Why use Gentarget’s lentivirus services? With > 20 year-experience in lentiviral technology, Gentarget provides the best services with Top Quality, Fastest Turnaround Time, and Best Price. As a leading lentivirus product provider worldwide, for more than 14 years, Gentarget are providing all kinds of lentivurs services, for hundred of academic and industrial customers. With thousands Read More >
CRISPR based epigenomic expression regulation
CRISPR/Cas9 gene editing has emerged as a powerful tool for making genomic sequence changes. However, concerns surrounding off-target effects and undesired alterations in the genome have been raised. To address these challenges, a mutant form of Cas9 known as dCas9 (deficient Cas9 or Dead Cas9) has been developed. Unlike Cas9, dCas9 lacks the ability to Read More >
Better In Vivo Imaging
Fluorescent markers and bioluminescence are two of the most commonly used methods for in vivo imaging in living cells. Fluorescence imaging is an extremely valuable tool, but it requires external excitation from a laser light. This laser light can have drawbacks such as autofluorescence, phototoxicity, and photobleaching. On the other hand, bioluminescence imaging does not require Read More >
COVID-19 Delta Variant and other mutations
As we are moving towards the end of 2021, people from all over the world are still impacted by the COVID-19 virus. We all are facing the challenge of the virus and its effects will still be visible for years to come. With the COVID-19 virus constantly undergoing variants such as the Delta variant and Read More >
SARS-CoV-2 Virus-Like Particles
Scheme of LentiVirus-Like Particles What is Virus-Like Particles (VLP)? A viral envelope is the outermost layer of the virus, consist of the glycoproteins. It serves to bind the receptor on the host’s cell membrane. When desired, the virus can be enveloped with a desired protein (so called pseudo-typing). The glycoproteins that able to maintain a Read More >
Stable Cell Line Express COVID-19 S Protein
There are many types of Coronaviruses which are common human pathogens. At early 2020, a novel coronavirus breakout in Wuhan China, and cause the current pandemic. On Feb 11, 2020, WHO named this virus as COVID-19 and the international Committee of Virus Taxonomy named it as SARS-Cov-2. Research found the COVID-19 bind human ACE2 receptor Read More >
When will the COVID-19 pandemic end?
The COVID-19 pandemic became a serve international public health threat. Huge efforts are put into developing the vaccines against COVID-19. Experts says the COVID-19 will not go away until an effective vaccine developed. Even with a “effective” vaccine, Scientists do not know for now, how long the vaccine immunity to COVID-19 virus. If the immunity Read More >
Live Visualization of Chromatin
What is Histone H2B? Histone H2B is one of the 4 main histone proteins involved in the structure of chromatin in eukaryotic cells. It has high level of positive charged amino acids at cellular pH, which allows it to bind negatively charged phosphate in DNA. It condenses chromatin to form a 30nm fiber, packaged in Read More >
Fluorescent-LC3 fusion for Autophagy Research
What is Autophagy? Autophagy is the lysosomal degradation route for soluble components of the cytosol and organelles. It helps to remove damaged organelles and misfolded or aggregated proteins. Cytoplasmic components become enclosed in a double membrane to form a compartment known as the autophagosome and are delivered to the lysosome for degradation. Autophagy failure is Read More >
COVID-19 vaccine, will it work?
How COVID-19 virus attacks human cells: The COVID-19 pandemic emerged as a severe threat to human health. It is caused by coronavirus 2 virus (SARS-CoV-2). Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The coronavirus surface presents a trimetric S protein consisting of N-terminal S1 and C-terminal Read More >
Fighting SARS-CoV-2 (COVID19)
ACE2 / TMPSS2 / S Protein: COVID19 virus attached human cells via two cell surface target, ACE2 and the serine protease, TMPRSS2, through its Spike (S) Protein. To facilitate the research for the treatment of COVID-19, Gentarget now provides the expression lentivirus products for human ACE2 (CAT#: LVP1310), TMPSS2 (CAT#: LVP1309), and Spike (S) (CAT#: Read More >
High Efficiency protein delivery
Engineered protein transmembrane domain (PTD) The direct introduction of protein into cells is a critical step in studying protein function. Unlike DNA transfection, in which the transcriptionally active DNA/gene is introduced into living cells where protein is made by cellular machinery, protein delivery does not introduce any exogenous DNA and is independent of the cellular Read More >
Enhanced super CMV promoter
Enhanced super CMV promoter RNA polymerase II is the main promoter category driven expression of thousands of genes. Its transcription mechanisms have been widely investigated. It involves many transcription factors and a combinatorial array of cis-regulatory DNA elements. Two parts were well characterized for transcription initiation complexes, the Core promoter and the Co-regulators. The core Read More >
PCR cloning -never been easier
PCR cloning kit Cloning is often limited by cost, labor-intensity, and incompatibility of restriction enzyme sites. Traditional in vitro cloning requires a variety of end-joining enzymes (e.g. T4 ligase, toposiomerase, blunt-end joining enzymes) or specific strand-exchange recombinases (e.g. CRE, loxP, Gateway BP, LR recombinase). Generating overhung- or blunt- ends requires either preparing vectors by restriction Read More >