Stem Cell Report
About GATA2 Promoter:
The GATA2 promoter drives a transcription factor, GATA2 gene which expresses at high levels in hematopoietic stem cell, and also in nonhematopoietic embryonic stem cells. GATA2 promoter also is active in the central nervous system but may not active or has very weak promoter strength in other cell types.
Lentivirus label Stem Cells:
GenTarget developed a set of reporting lentivirus for specifically or preferably labeling Stem Cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the human GATA2-promoter (click to see its sequence) that highly expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. Those reporter lentivirus are best suitable for infecting the hematopoietic and embryonic Stem Cells, as well as for the signal pathway research on GATA2 promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
see Product Manual for details (.pdf).