Ovarian Cancer Reporter

HE4  Promoter: The human epididymis protein 4 (HE4) is a tumor marker of ovarian cancer. It is a s Secreted glycoprotein that over-expressed by serous and endometrioid ovarian carcinomas. HE4 promoter is highly transcriptionally active in ovarian cancer cells and used in transcriptional targeting in ovarian cancer gene therapy.

GenTarget developed a set of reporting lentivirus for selectively labeling ovarian cancer cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human HE4  gene (click to see its sequence) that highly expressed in ovarian cancer cells and some other cancers. Those reporter lentivirus are best suitable for infecting the human or mouse ovarian cancer cells, as well as for the signal pathway research on HE4  promoter regulation.

Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. It also  provides internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter (wherever the RSV promoter is active in assay cell type). See the scheme below for lentivector’s core expression cassette.

The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse ovarian cancer cells in vitro and in vivo via the luciferase signal or fluorescent signal.

The premade, ready-to-use reporter lentivirus provides in two formats: 200ul in DMEM medium with 10% FBS and 10x polybrene, or 200ul concentrated virus in PBS solution. See each product below, or click to see Product Manual for details (.pdf).