Skip to content

For product queries: Call 1-858-265-6446 or email at info@gentarget.com

| Login / Register | 0 items | $0.00

gentarget inc.

7930 Arjons Drive, Suite B
San Diego, CA 92126
United States

  • Home
  • Products
    • Premade Lentivirus
      • cDNA (ORF) Expression
      • Luciferases
      • Fluorescent Markers
      • Sub-cellular Imaging
      • Luminescent Imaging
      • Cytoskeleton Imaging
      • Unstable GFP
      • near-infrared RFP
      • Fluorescent fusion
      • CRE recombinase
      • LoxP GFP/RFP ColorSwtich
      • SEAP Reporter
      • Tet Repressor
      • rtTA Expression
      • lacZ Enzyme
      • iPS stem cell factors
      • Other enzyme
    • Pathway Reporter
      • Androgen Pathway
      • Antioxidant Pathway
      • C/EBP Pathway
      • CD44CR1 Pathway
      • CREB (cAMP-PKA) Pathway
      • EGR1 Promoter Pathway
      • Estrogen Receptor Pathway
      • Glucocorticoid Pathway
      • Hedgehog Pathway
      • Hypoxia Pathway
      • JAK-STAT Pathway
      • JNK / AP1 Pathway
      • MAPK/ERK Pathway
      • Notch Signal Pathway
      • NFκB Pathway
      • TP53 Pathway
      • Wnt Signal pathway
      • Blue light inducible reporter
      • Heat inducible expression
      • Mitochondrial pH-sensitive probe
      • Signal Pathway Control
    • Cell Immortalization
      • hTERT Expression
      • SV40 Large T antigen
      • P53 siRNA Lentivirus
      • Other Immortalization Factors
    • ImmunoOncology Research
      • CAR-T and More
      • Assay Cell Lines
      • Cell Antigens & Receptors
    • CRISPR Gene Editing
      • Cas9 Expression
      • dCas9 Expression
      • gRNA Lentivirus
      • CRISPR Repair & KnockIn
      • CRISPR KnockOut
    • Stable cell lines
      • Pathway Cell Line
      • Luciferase cells
      • Fluorescent cells
      • CRE reporting cells
      • CRE Expression Cells
      • CRISPR ready cell lines
      • TetR stable cells
      • rtTA stable cells
      • Inducible expression cell lines
      • Ion Channel cells
      • LacZ stable cells
      • Other stable cells
    • Cell-Specific Reporter
      • Astrocytes Report
      • B-Cell Reporter
      • Brain Cell Report
      • Cancer Specific Report
      • Endothelial Cell Report
      • Hematopoietic Cell Report
      • Hepatoma Cell Report
      • Kidney Cell Report
      • Leukocytes Report
      • Lung Tissue Report
      • Macrophages Report
      • Megakaryocytes Report
      • Monocytes Report
      • Muscle Cell Report
      • Neuron Report
      • Ovarian Cancer Report
      • Pancreatic Cancer Report
      • Prostate Cell Report
      • Stem Cell Report
    • Infectious Antigens
      • Viral Antigens
    • Virus Like Particles (VLP)
    • Non-integrating LV
    • Adenovirus
      • Common Markers & Enzymes
      • GFP CFP YFP RFP BFP & Controls
      • iPS Stem Cell Factors
      • ORF Expression
    • shRNA lentivirus
      • Validated shRNA Lentivirus
      • Customized shRNA
    • microRNA lentivirus
    • anti miRNA lentivirus
    • Lentivectors
      • CRISPR gRNA lentivectors
      • shRNA Lentivectors
      • Promoter-less Lentivectors
      • Lentivirus Packaging plasmids
    • Control lentivirus
    • Ultra titer lentivirus
    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
  • Services
    • Lentivirus Service
    • Customized Stable Cell Lines
    • Fusion In Vivo PCR Cloning
    • Protein Expression
    • Antibody Cloning and Expression
  • Support
    • Technology
      • Optional Inducible Lentiviral System
      • SureTiter™ Lentiviral System
      • Enhanced super CMV promoter
      • InTag™ trans-membrane proteins
      • Fusion in vivo, Eco™ PCR Cloning
    • Protocols
    • MSDS Files
    • Promotion
    • Product Manuals
    • Vector sequences
    • Product FAQs
    • Product citations
    • Terms
    • Useful Links
  • Distributors
  • About Us
  • Contact
  • News
Home / Pathway Reporter / Hedgehog Pathway

Hedgehog Pathway

About Hedgehog Pathway:

The hedgehog pathway involves stem cell growth, many type tumor growth and CNS development. The pathway is equally important during vertebrate embryonic development. The pathway takes its name from its polypeptide ligand, an intercellular signaling molecule called Hedgehog. The mammalian hedgehog has three homologues, Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh).  The Hedgehog binds to their membrane receptor, activating the Gli proteins (transcription factors). The Gli proteins bind to Gli responsive element (Gli-RE) sequence in target’s promoter region, which leads to hedgehog signal’s target gene expression.

Detect Hedgehog Pathway:

GenTarget developed a set of reporting lentivirus to measure the activation of Hedgehog signal pathway.  Those reporting lentivirus has a luminescent report (Luciferase, Renilla Luc) or a fluorescent report (GFP, RFP) or a  secreted SEAP report,  under a minimal CMV promoter (mCMV) that embedded with optimized tandem repeats of Gli responsive element sequence (5′-GACCACCCAC). When Gli proteins bind to Gli responsive element sequence, the downstream reporter is expressed as the result of activation for the minimal promoter. The reporter’s signal can be easily and rapidly readout via plate assays (luciferase assay or by fluorescent microscope).

The luciferase signal can be measured via Luciferase assay. The fluorescent reporter can be detected via its fluorescent signal. The SEAP secreted into cell culture supernatant, which allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies.

Use Hedgehog Pathway Report Lentivirus:

Those reporting lentivirus contains a constitutively expressed fluorescent selection marker or an antibiotic selection marker under the RSV promoter, which makes it easier to select the stably infected signal reporting cells (to generate pathway specific sensor cell lines), or provides internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter. See the lentivector core expression cassette scheme below.

Hedgehog pathway lentivector map

The premade, ready-to-use reporter lentivirus provides a much easier tool to monitor the activity of Hedgehog signaling pathways in virtually any mammalian cell type. It also allows to generate your own reporting cell line in your desired cell type for study or screen of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment (such as Shh protein) in the cell based assay.

Click the Product Manual (pdf) for all available pathway reporter products.

NameSKUPriceBuy
Gli-GFP (Bsd) lentivirusLVP945-B$495.00
Gli-GFP (Bsd) lentivirus in PBSLVP945-B-PBS$650.00
Gli-GFP (Neo) lentivirusLVP945-N$495.00
Gli-GFP (Neo) lentivirus in PBSLVP945-N-PBS$650.00
Gli-GFP (Puro) lentivirusLVP945-P$495.00
Gli-GFP (Puro) lentivirus in PBSLVP945-P-PBS$650.00
Gli-GFP (RFP) lentivirusLVP945-R$495.00
Gli-GFP (RFP) lentivirus in PBSLVP945-R-PBS$650.00
Gli-Luc (Bsd) lentivirusLVP947-B$495.00
Gli-Luc (Bsd) lentivirus in PBSLVP947-B-PBS$650.00
Gli-Luc (GFP) lentivirusLVP947-G$495.00
Gli-Luc (GFP) lentivirus in PBSLVP947-G-PBS$650.00
Gli-Luc (Neo) lentivirusLVP947-N$495.00
Gli-Luc (Neo) lentivirus in PBSLVP947-N-PBS$650.00
Gli-Luc (Puro) lentivirusLVP947-P$495.00
Gli-Luc (Puro) lentivirus in PBSLVP947-P-PBS$650.00
Gli-Luc (RFP) lentivirusLVP947-R$495.00
Gli-Luc (RFP) lentivirus in PBSLVP947-R-PBS$650.00
Gli-RFP (Bsd) lentivirusLVP946-B$495.00
Gli-RFP (Bsd) lentivirus in PBSLVP946-B-PBS$650.00
Gli-RFP (GFP) lentivirusLVP946-G$495.00
Gli-RFP (GFP) lentivirus in PBSLVP946-G-PBS$650.00
Gli-RFP (Neo) lentivirusLVP946-N$495.00
Gli-RFP (Neo) lentivirusLVP946-N-PBS$650.00
Gli-RFP (Puro) lentivirusLVP946-P$495.00
Gli-RFP (Puro) lentivirus in PBSLVP946-P-PBS$650.00
Gli-RLuc (Bsd) lentivirusLVP948-B$495.00
Gli-RLuc (Bsd) lentivirus in PBSLVP948-B-PBS$650.00
Gli-RLuc (GFP) lentivirusLVP948-G$495.00
Gli-RLuc (GFP) lentivirus in PBSLVP948-G-PBS$650.00
Gli-RLuc (Neo) lentivirusLVP948-N$495.00
Gli-RLuc (Neo) lentivirus in PBSLVP948-N-PBS$650.00
Gli-RLuc (Puro) lentivirusLVP948-P$495.00
Gli-RLuc (Puro) lentivirus in PBSLVP948-P-PBS$650.00
Gli-RLuc (RFP) lentivirusLVP948-R$495.00
Gli-RLuc (RFP) lentivirus in PBSLVP948-R-PBS$650.00
Gli-SEAP (Bsd) lentivirusLVP1269$495.00
Gli-SEAP (Bsd) lentivirus in PBSLVP1269-PBS$650.00
Gli-SEAP (Neo) lentivirusLVP1270$495.00
Gli-SEAP (Neo) lentivirus in PBSLVP1270-PBS$650.00
Gli-SEAP (Puro) lentivirusLVP1268$495.00
Gli-SEAP (Puro) lentivirus in PBSLVP1268-PBS$650.00
Gli-SEAP (RFP) lentivirusLVP1271$495.00
Gli-SEAP (RFP) lentivirus in PBSLVP1271-PBS$650.00

    Cart

    Product Selection Guides

    • Luciferase Imaging
    • Fluorescent Markers
    • Sub-cellular Imaging
    • Signal Pathway Assay
    • CRISPR Gene Editing
    • ImmunoOncology Research
    • Cell Immortalization
    • Inducible Expression
    • Reporter Cell Lines
    • Promoter Research
    • iPS Factors

    VIEW MORE

    Stay Connected

    Online Payment Service
  • Home
  • Products
    • Premade Lentivirus
    • Pathway Reporter
    • Cell Immortalization
    • ImmunoOncology Research
    • CRISPR Gene Editing
    • Stable cell lines
    • Cell-Specific Reporter
    • Infectious Antigens
    • Virus Like Particles (VLP)
    • Non-integrating LV
    • Adenovirus
    • shRNA lentivirus
    • microRNA lentivirus
    • anti miRNA lentivirus
    • Lentivectors
    • Control lentivirus
    • Ultra titer lentivirus
    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
  • Services
    • Lentivirus Service
    • Customized Stable Cell Lines
    • Fusion In Vivo PCR Cloning
    • Protein Expression
    • Antibody Cloning and Expression
  • Support
    • Technology
    • Protocols
    • MSDS Files
    • Promotion
    • Product Manuals
    • Vector sequences
    • Product FAQs
    • Product citations
    • Terms
    • Useful Links
  • Distributors
  • About Us
  • Contact
  • News

2008 ~ ©, 2023 GenTarget Inc. All rights reserved.

Contact | Terms | View Cart

Scroll Up