Hematopoietic Cell Report
About CD45 Promoter:
PTPRC (Protein tyrosine phosphatase, receptor type, C) is also known as CD45 antigen which was originally called leukocyte common antigen (LCA). CD45 is a high molecular weight, transmembrane protein-tyrosine phosphatase expressed on all nucleated cells of hematopoietic origin. CD45’s promoter is active only after ES cells differentiate into hematopoietic cell and active exclusively in all hematopoietic cells. The CD45 promoter does not contain an apparent TATA box or CCAAT box.
The spleen focus-forming virus (SFFV) promoter is derived from the SFFV retrovirus and is known for its strong and constitutive activity in a wide range of cell types. It is often used in gene therapy constructs to drive high levels of gene expression in hematopoietic stem cells.
Lentivirus Report for Hematopoietic Cells:
GenTarget developed a set of report lentivirus specifically label Hematopoietic cells. Those report lentivirus express a luminescent or a fluorescent report under the promoter of human CD45 gene (click to see its sequence) or SFFV promoter, for high expression in Hematopoietic cells.
Those report lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. The lentivector’s core expression cassette is showed in scheme below:
See each product below, or click to see Product Manual for details (.pdf).