Hematopoietic Cell Report
About CD45 Promoter:
PTPRC (Protein tyrosine phosphatase, receptor type, C) is also known as CD45 antigen which was originally called leukocyte common antigen (LCA). CD45 is a high molecular weight, transmembrane protein-tyrosine phosphatase expressed on all nucleated cells of hematopoietic origin. CD45’s promoter is active only after ES cells differentiate into hematopoietic cell and active exclusively in all hematopoietic cells. The CD45 promoter does not contain an apparent TATA box or CCAAT box.
Lentivirus Report for Hematopoietic Cells:
GenTarget developed a set of report lentivirus specifically label Hematopoietic cells. Those report lentivirus express a luminescent or a fluorescent report under the promoter of human CD45 gene (click to see its sequence) that specific expressed in Hematopoietic cells.
Those report lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
The premade,ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or imaging of human or mouse Hematopoietic cells in vitro and in vivo via the luciferase signal or fluorescent signal.
See each product below, or click to see Product Manual for details (.pdf).