Hematopoietic Cell Reporter

CD45 Promoter: PTPRC (Protein tyrosine phosphatase, receptor type, C) is also known as CD45 antigen which was originally called leukocyte common antigen (LCA). CD45 is a high molecular weight, transmembrane protein-tyrosine phosphatase expressed on all nucleated cells of hematopoietic origin. CD45’s promoter is active only after ES cells differentiate into hematopoietic cell and active exclusively in all hematopoietic cells. The CD45 promoter does not contain an apparent TATA box or CCAAT box.

GenTarget developed a set of reporting lentivirus for specifically labeling Hematopoietic cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human CD45 gene (click to see its sequence) that specific expressed in Hematopoietic cells. Those reporter lentivirus are best suitable for infecting the human or mouse Hematopoietic cells, as well as for the signal pathway research on CD45 promoter regulation.

Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. It also  provides internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter (wherever the RSV promoter is active in assay cell type). See the scheme below for lentivector’s core expression cassette.

The premade,ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse Hematopoietic cells in vitro and in vivo via the luciferase signal or fluorescent signal.

The premade, ready-to-use reporter lentivirus provides in two formats: 200ul in DMEM medium with 10% FBS and 10x polybrene, or 200ul concentrated virus in PBS solution. See each product below, or click to see Product Manual for details (.pdf).