Introduction:

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein.  The most well-known luciferases are from the firefly Luciferase (F-Luc) from Photinus pyralis and the sea pansy Renilla (R-Luc) reniformis, and a red-shifted luciferase (Red-Luc). Some luciferases are naturally secreted, such as Gaussia luciferase and Cypridina luciferase.  Nano-luciferase (N-Luc) is a newly engineered luciferase enzyme with much bright signal.

The luciferase signal can be easily detected, and luciferase enzymatic assays are widely used because of their high sensitivity, high linear dynamical ranges, and high through-put assay adaptation.

Properties of Different Luciferase Enzyme:

Product Features:

GenTarget luciferase expression lentivirus containing wide selection of fluorescent markers, antibiotic markers, or fluorescent-antibiotic fusion dual markers. The different luciferase is expressed under  the different promoter (CMV, EF1a, CAG, mPGK, Ubc, Actin-beta) to best fit different cell types.  see lentivector core structure scheme below.

GenTarget’s Luciferase Expression lentiviruses are provided as 200 µl/per vial in two formats:   

1. DMEM medium with 10 % FBS and 60 µg/ml polybrene (10x)
2. PBS solution, which is best for in vivo applications, cell cultures requiring serum-free conditions, or for hard-to-infect cells. 

 Note: Ultra concentrated virus (>= 10⁹ IFU/ml) is available upon request.

 Or click Product Manual for details (.pdf).