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Home / shRNA lentivirus / Validated shRNA Lentivirus

Validated shRNA Lentivirus

Knockdown Validated shRNA Lentivirus:

GenTarget’s premade Lentiviral Particles are the most advanced tools available for delivering target over-expression or knockdown into a wide range of cell types including primary and non-dividing cells. The pre-made shRNA lentivirus demonstrates greater than 75~95% knockdown levels validated in in a reporter assay where the specific target was fusioned with lacZ or luciferase reporter. The knockdown levels were reflected by decreases of lacZ or luciferase activity. 

Applications: 

    1. Delivery of  expression knockdown into hard-to-transfect cell types (such as neuronal cells) without the use of transfection reagents
    2. Creation of knockdown stable cell lines for long-term study
    3. Knockdown of target in primary or drug-arrested cells 

Product Description: 

Each shRNA lentiviral particles (or lentivirus) is guaranteed to knockdown the specified endogenous target. The H1 driven shRNA lentivirus can be used for constitutive, or optionally for tetracycline- inducible knockdown. The U6 driven shRNA are used for constitutive knockdown. They contain an antibiotic marker or a “fluorescent-antibiotic” fusion dual marker (GFP or RFP fusion with puromycin or blasticidin) for both antibiotic selection and/or fluorescence sorting. See the shRNA core map scheme below.

shRNA expression lentivector map

Questions?  Please see FAQs for pre-made lentiviral particles. 

Please click  here for Transduction protocols for adhesive and suspension cells. 

Features of validated shRNA lentivirus: 

    1. Easy-to-use: directly add particles to cells. There is no need for any lipids or transfection reagents
    2. 75% to 95% knockdown, depending on cell types
    3. Optional inducible shRNA expression: Particles can be used for constitutive knockdown or for Tet- inducible shRNA expression in the presence of TetR (for H1 promoter driven shRNA only).
    4. Safety: Self-inactivation prevents replication of the virion
    5. High true titer:  at 1 x 107 IFU/ml in DMEM medium, or  at 5 x 107 IFU/ml in PBS;
    6. Dual selection markers: easy real-time monitoring of target expression by fluorescence and antibiotic selection of positive transduced cells with puromycin or blasticidin  

Custom shRNA: 

Not find the shRNA for your gene? No problems. We also provide Custom shRNA Services to generate your specific shRNA lentiviral particles. Please contact us for a service quote!

Please click each product below and click into each Product Manual for details. 

NameSKUPriceBuy
shRNA (h P53)-(GFP-Bsd) lentivirusLVP343-GB$295.00
shRNA (h P53)-(GFP-Bsd) lentivirus in PBSLVP343-GB-PBS$650.00
shRNA (h P53)-(GFP-Puro) lentivirusLVP343-GP$295.00
shRNA (h P53)-(GFP-Puro) lentivirus in PBSLVP343-GP-PBS$650.00
shRNA (h P53)-(RFP-Bsd) lentivirusLVP343-RB$295.00
shRNA (h P53)-(RFP-Bsd) lentivirus in PBSLVP343-RB-PBS$650.00
shRNA (h P53)-(RFP-Puro) lentivirusLVP343-RP$295.00
shRNA (h P53)-(RFP-Puro) lentivirus in PBSLVP343-RP-PBS$650.00
shRNA (lacZ)-(GFP-Bsd) lentivirusLVP344-GB$295.00
shRNA (lacZ)-(GFP-Bsd) lentivirus in PBSLVP344-GB-PBS$650.00
shRNA (lacZ)-(GFP-Puro) lentivirusLVP344-GP$295.00
shRNA (lacZ)-(GFP-Puro) lentivirus in PBSLVP344-GP-PBS$650.00
shRNA (lacZ)-(RFP-Bsd) lentivirusLVP344-RB$295.00
shRNA (lacZ)-(RFP-Bsd) lentivirus in PBSLVP344-RB-PBS$650.00
shRNA (lacZ)-(RFP-Puro) lentivirusLVP344-RP$295.00
shRNA (lacZ)-(RFP-Puro) lentivirus in PBSLVP344-RP-PBS$650.00
shRNA (Luc)-(GFP-Bsd) lentivirusLVP345-GB$295.00
shRNA (Luc)-(GFP-Bsd) lentivirus in PBSLVP345-GB-PBS$650.00
shRNA (luc)-(GFP-Puro) lentivirusLVP345-GP$295.00
shRNA (luc)-(GFP-Puro) lentivirus in PBSLVP345-GP-PBS$650.00
shRNA (luc)-(RFP-Bsd) lentivirusLVP345-RB$295.00
shRNA (luc)-(RFP-Bsd) lentivirus in PBSLVP345-RB-PBS$650.00
shRNA (luc)-(RFP-Puro) lentivirusLVP345-RP$295.00
shRNA (luc)-(RFP-Puro) lentivirus in PBSLVP345-RP-PBS$650.00
shRNA-H1(Neg)-(GFP-Bsd) lentivirusH1(shRNA-Ctr)-GB$295.00
shRNA-H1(Neg)-(GFP-Bsd) lentivirus in PBSH1(shRNA-Ctr)-GB-PBS$650.00
shRNA-H1(Neg)-(GFP-Puro) lentivirusH1(shRNA-Ctr)-GP$295.00
shRNA-H1(Neg)-(GFP-Puro) lentivirus in PBSH1(shRNA-Ctr)-GP-PBS$650.00
shRNA-H1(Neg)-(RFP-Bsd) lentivirusH1(shRNA-Ctr)-RB$295.00
shRNA-H1(Neg)-(RFP-Bsd) lentivirus in PBSH1(shRNA-Ctr)-RB-PBS$650.00
shRNA-H1(Neg)-(RFP-Puro) lentivirusH1(shRNA-Ctr)-RP$295.00
shRNA-H1(Neg)-(RFP-Puro) lentivirus in PBSH1(shRNA-Ctr)-RP-PBS$650.00

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  • Home
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    • Premade Lentivirus
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    • shRNA lentivirus
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    • Lentivectors
    • Control lentivirus
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    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
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