Hepatoma Cell Report
About αFP Promoter:
Alpha-fetoprotein (aFP) a major plasma protein produced in liver during fetal development. aFP is normally expressed in fetal but not adult livers. aFP will be elevated in some of diseases such as Hepatocellular carcinoma/ hepatoma. The aFP promoter has been shown to confer selective expression of a transgene. The expression level of the transgene is proportional to the level of aFP expression in the transfected cells. Thus, aFP promoter driven a reporter is highly expressed in hepatocellular carcinoma (HCC) cells.
Report Lentivirus for Hepatoma Cells:
GenTarget developed a set of reporting lentivirus for specifically labeling hepatocellular carcinoma cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human aFP gene (click to see its sequence) that specifically expressed in hepatocellular carcinoma cells. Those reporter lentivirus are best suitable for infecting the human or mouse hepatocellular carcinoma, as well as for the signal pathway research on aFP promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells via antibiotic killing or fluorescent cell sorting.
See the scheme below for lentivector core expression cassette.
see Product Manual for details (.pdf).