Endothelial Cell Reporter
FLT1 Promoter: Human FLT1 gene encodes Vascular endothelial growth factor receptor 1 (VEGFR1) which is one of the receptors for VEGF. It contains an extracellular ligand-binding region, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. Flt-1 is expressed specifically in endothelial cells and up-regulated in tumor vasculature. Its promoter region contains a TATA box, a GC-rich region, nine Ets motifs and one CREB/ATF motif.
GenTarget developed a set of reporting lentivirus for specifically labeling Endothelial Cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human FLT1 gene (click to see its sequence) that specific expressed in Endothelial Cells. Those reporter lentivirus are best suitable for infecting the human or mouse in Endothelial Cells, as well as for the signal pathway research on FLT1 promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. It also provides internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter (wherever the RSV promoter is active in assay cell type). See the scheme below for lentivector’s core expression cassette.
The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse Endothelial Cells in vitro and in vivo via the luciferase signal or fluorescent signal.
The premade, ready-to-use reporter lentivirus provides in two formats: 200ul in DMEM medium with 10% FBS and 10x polybrene, or 200ul concentrated virus in PBS solution. See each product below, or click to see Product Manual for details (.pdf).