Estrogen Receptor Pathway
About Estrogen Receptor Pathway:
Estrogen receptors (ERs) are steroid hormone receptors important in development, growth, and reproduction. ERs play a large role in female organ cancers, especially breast cancer. ERs are activated by the hormone estrogen. Two classes of ER exist: nuclear estrogen receptors (ERα and ERβ) and membrane estrogen receptors (mERs), the mostly G protein-coupled receptors. Once activated by estrogen, the ER is able to translocate into the nucleus and bind to specific oestrogen receptor response elements (ER-RE), to regulate the activity of different genes. As hormone receptors, ERs are important in sexual maturation and gestation, including normal cell growth, and the development of the reproductive, central nervous and skeletal systems and cancel development.
Detect Estrogen Pathway Activity:
GenTarget developed a set of reporting lentivirus to monitor the activity of Estrogen-receptor regulated signal transduction pathways in cultured cells. Those reporting lentivirus has a luminescent report (Luciferase, Renilla Luc) or a fluorescent report (GFP, RFP) or a secreted SEAP report, under a minimal promoter that embedded with optimized tandem repeats of estrogen receptor response element (ER-RE) sequence motif (5′- AGGTCAcagTGACCT). When estrogen receptor binds to estrogen receptor-RE sequence, the downstream reporter is expressed as the result of activation for the minimal CMV promoter (mCMV).
The luciferase signal can be measured via Luciferase assay. The fluorescent reporter can be detected via its fluorescent signal. The SEAP secreted into cell culture supernatant, which allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies.
Estrogen Receptor Pathway Lentivirus:
Those reporting lentivirus also contains a constitutively expressed fluorescent selection marker or an antibiotic selection marker under the RSV promoter, which makes it easier to select the stably infected signal reporting cells (to generate pathway specific sensor cell lines), or provides internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter. See core lentivector cassette below.
The premade, ready-to-use reporter lentivirus provides a much easier tool to monitor the activity of estrogen receptor regulated signaling pathways in virtually any mammalian cell type. It also allows to generate your own reporting cell line in your desired cell type for study or screen of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment in the cell based assay.
Click the Product Manual (pdf) for all available product for this pathway reporter.