“Fluorescent-Target” Fusion Expression Lentivirus:

Those product express the “fluorescent-Target” fusion proteins. A desired target is fused to Green, Blue, Red, or Cyan Fluorescent Protein, demonstrating strong Fluorescent signal coupled with the target’s functionality.

The target gene is in frame with a fluorescent marker,  expressed as a covalent bond fusion protein under a proprietary suCMV promoter that demonstrates the highest expression levels (3-10 fold higher than the CMV promoter in the pCDNA6.3 vector, depending upon cell type).  Each fluorescent tag is codon optimized to generate the brightest signal.

An antibiotic selection marker (Puromycin, Blasticidin or Neomycin) is expressed under the RSV promoter. See map scheme below.

Gentarget’s Fluorescent-target fusion lentivirus are the best in the class, demonstrating the brightest fluorescent signal, strong transduction efficiency. .

Lentivirus are provided in DMEM medium with 10% FBS and 60 ug/ml polybrene (10x), or concentrated and resuspended into PBS solution, as ready to use status. Note: higher titer viruses are available upon special requests.

Key features:

• Robust Expression and High Titer: GenTarget’s Premade Lentiviral Particles have the brightest fluorescence and the strongest transduction efficiency of any lentiviral particles on the market.
• Long-Term Expression: GenTarget’s Premade Lentiviral Particles produce long-lasting expression of fluorescently-labeled target proteins even in hard-to-transfect cell lines such as primary and neuronal cells.
• Easy Transduction: Simply add the Particles to your cell culture and visualize fluorescence in 48-92 hours. There is no need for any additives or changes of medium.
• Multiple Colors: Particles expressing different colors may be transduced into the same cells for multi-color applications.
• Easy Selection of Transduced Cells:  Use either fluorescence signal or puromycin / Blasticidin / Neomycin resistance.
• Tested and Validated: Each lot of Particles is validated and guaranteed to be of the highest quality.
• Ready-to-Use:  Particles are provided in DMEM medium with 10 % FBS and 60 µg/ml polybrene (10x).

For general questions about our ready-to-use lentiviral particles, please See FAQ for pre-made lentiviral particles (.pdf)

If you want to label your specific target, Gentarget Inc also provides lentiviral services for making the fusion protein of your gene of interest and a fluorescent marker, and generates ready-to-use viral particles with the best prices and fastest turnaround time.

Please click links below for each product’s details, or click here for Product Manual.