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Home / Stable cell lines / CRE reporting cells

CRE reporting cells

About CRE-LoxP for GFP/RFP color switch:

Those reporting cell lines, derived from HEK293 cells, constitutively expresses a “LoxP-GFP-Stop-LoxP-RFP-Stop” cassette under strong suCMV promoter. See the genomic integrated express cassette structure below.

LoxP-GFP/RFP color switch lentivector

When CRE enzyme is present, CRE-LoxP recombination will excise out the GFP region between two loxP sites, resulting GFP signal disappearing and RFP signal being turned on in the cells.  

The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the CRE recombination events ( by your CRE expression plasmids, or  CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system.

Gentarget developed three CRE reporting Cell lines containing different antibiotic selection maker, Puromycin, Neomycin or Blasticidin. see details in Product Manual.

Product Citations:

  1. ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrp rd6 mouse;
  2. In vivo screening identifies GATAD2B as a metastasis driver in KRAS-driven lung cancer;
NameSKUPriceBuy
HEK293-loxP-GFP-RFP (Bsd)SC018-Bsd$3,000.00
HEK293-loxP-GFP-RFP (Neo)SC018-Neo$3,000.00
HEK293-loxP-GFP-RFP (Puro)SC018-Puro$3,000.00
HeLa-loxP-GFP-RFP (Neo)SC071-Neo$3,000.00
HeLa-loxP-GFP-RFP (Puro)SC071-Puro$3,000.00

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  • Products
    • Premade Lentivirus
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    • Cell Immortalization
    • ImmunoOncology Research
    • CRISPR Gene Editing
    • Stable cell lines
    • Cell-Specific Reporter
    • Infectious Antigens
    • Virus Like Particles (VLP)
    • Non-integrating LV
    • Adenovirus
    • shRNA lentivirus
    • microRNA lentivirus
    • anti miRNA lentivirus
    • Lentivectors
    • Control lentivirus
    • Ultra titer lentivirus
    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
  • Services
    • Lentivirus Service
    • Customized Stable Cell Lines
    • Fusion In Vivo PCR Cloning
    • Protein Expression
    • Antibody Cloning and Expression
  • Support
    • Technology
    • Protocols
    • MSDS Files
    • Promotion
    • Product Manuals
    • Vector sequences
    • Product FAQs
    • Product citations
    • Terms
    • Useful Links
  • Distributors
  • About Us
  • Contact
  • News

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