for Human, Mouse and Rat cDNAs:

All lentivirus in this category were generated using GenTarget’s tetracycline Optional Inducible Lentiviral System. Each gene was fully sequence verified and 100% matched to individual NCBI accession IDs at the coding DNA sequence (CDS). 

All particles are pre-made, in stock, ready-to-ship, provided as 200µl /per vial in DMEM medium, or concentrated virus in PBS (Note: shipped in dry-ice package via next day express). 

Each target wes expressed under the optional inducible TetCMV promoter. A RFP-Bsd or GFP-Puro fusion dual marker under RSV promoter allows sorting on RFP or GFP, or selection via antibiotic-killing. RFP / GFP signal also provides  convenient, real time monitoring of the particles’ performance. See vector scheme below:

Advanced Feature: GenTarget’s target expression lentivirus can be used for regular constitutive expression. However, when inducible expression is desired, they can be used for inducible expression in the presence of a repressor protein (tetR). For inducible expression, the target expression is first repressed by the repressor protein (TetR) then induced by addition of tetra acycline.

The presence of TetR can be achieved by co-transfection with TetR expressing lentivirus. Please see “FAQ about premade lentiviral particles” for more information. Please see Product manual for product details. 

GenTarget also provides  Negative control lentivirus for controlling for the effects of virus infection upon a given cell line, which validates the specificity of any target expression effects. 

Pre-made over-expression lentiviruses are easy to use.  Simply add the virus to your cell culture and wait 3 days for the expression peak. Please see “transduction protocols” for the application method. 

Please check Product List file (.pdf) for all available target expression lentiviurs.

You can also search by gene symbol or accession ID (NM_xxxxxx) on the home page. If you do not find the gene of your interest, we can generate the ready-to-use expression lentivirus through a  Service Project.