Ion Channel cells
Human Ion-Channel stable cell lines:
The human ion-channel stable cell lines are derived from the adhesive enhance HEK293 cells, transformed by the expression lentivirus expressing a well characterized human ion-channel target.
The expression cassette (see the structure scheme below) was stably integrated into HEK293 cells. Each human ion-channel target’s codon sequence is natively expressed (without any tags) under an enhanced EF1a promoter. The cell lines also have a RFP–blasticidin, (fluorescent-antibiotic) fusion dual marker under RSV promoter. Therefore, each cell also demonstrates RFP signal which can be visualized under fluorescent microscope.
The integrated expression cassette in ion-channel stable cells:
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