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Home / CRISPR Gene Editing / Cas9 Expression

Cas9 Expression

About CRISPR-Ca9:

Cas9 (CRISPR associated protein 9) is endonuclease that can cut the double-strand DNA, and thereby alters a cell’s genome. CRIAPS-Ca9 genomic gene-editing technology utilizes a guide RNA molecule (gRNA) to direct a Cas endonuclease to the genomic DNA targeted site. Then, the Cas enzyme creates a double-strand break that can be repaired either by Non-Homologous End-Joining (NHEJ), which can result in insertion or deletions (InDels), or by Homology Directed Repair (HDR) when a repair Donor DNA template is present.

InDels can disrupt the expression of the target gene (So-called, KnockOut). While repaired by HDR, the genome was modified with a foreign gene (So-called, KnockIn), or with sequence corrected gene (targeted gene therapy or gene repair).

Cas9 (from Streptococcus pyogenes ) is the most frequently used cas endonuclease in CRISPR. Ca9 protein can be delivered as the standalone expression, or delivered with a target gRNA in a one-vector system.

GenTarget offers standalone Cas9  Expression Lentivirus, and other modified Cas9 enzymes. To avoid the unwanted permanent Cas9 expression, Gentarget also provides the Ca9 Expression Adenovirus, and the Integration-Deficient Lentivirus for Cas9 expression.

Cas9 Expression Products:

Our Cas9 lentivirus are produced from our proprietary high-titer lentivectors that express a nuclear penetrating humanized wild-type Cas9 endonuclease (Streptococcus pyogenes). The Cas9 enzyme is driven by different promoters with a variety of antibiotic selection markers (see the core expression lentivector scheme below), providing you an easy delivery for cas9 expression in almost all cell types, including the hard-to-transfected cell types, primary cells, and non-dividing cells, which makes the gene-editing possible in all cell types.

Cas9-expression-scheme

Cas9 Lentivirus Product Features:

  1. High Cas9 expression Level by enhanced promoters to fit different cell types;
  2. High titer lentivirus provides more efficient Cas9 delivery in almost all cell types including primary cells and non-dividing cells;
  3. Flexible selection with different antibiotic and fluorescent markers;
  4. Best nuclear penetrating with enhanced NLS leader: the Cas9 is expressed with an optimized, proprietary Nuclear Localization Signal (NLS), providing the efficient cas9 delivery into the nuclear region where the gene-editing occurs.
  5. Easy to use: you can simply synthesize the gRNA (or use gRNA lentivirus) and used it together with the Cas9 lentivirus for targeted gene editing.
  6. The standalone Cas9 lentivirus allows generating CRISPR-ready animal models or stable cell lines in your desired cell types or animals that are used as a model for any gene editing.

Please see Cas9 Lentivirus Product Manual, or see each product detail below.

NameSKUPriceBuy
CAG-h Cas9 (Neo) lentiviral particles, in vivo readyLVP689-PBS$650.00
Cas9 (CAG, Bsd) Expression Lentivirus, ConcentratedLVP688-PBS$650.00
Cas9 (CAG, Puro) Expression Lentivirus, ConcentratedLVP687-PBS$650.00
Cas9 (CMV, Bsd) Expression Lentivirus, concentratedLVP682-PBS$650.00
Cas9 (CMV, GFP-Bsd) Expression Lentivirus, ConcentratedLVP680-PBS$650.00
Cas9 (CMV, GFP-Puro) Expression Lentivirus, ConcentratedLVP678-PBS$650.00
Cas9 (CMV, Neo) Expression Lentivirus, ConcentratedLVP683-PBS$650.00
Cas9 (CMV, No Selection) lentiviral particles, in vivo readyLVP1393-PBS$650.00
Cas9 (CMV, Puro) lentiviral particles, in vivo readyLVP681-PBS$650.00
Cas9 (CMV, RFP-Bsd) Expression Lentivirus, ConcentratedLVP707-PBS$650.00
Cas9 (CMV, RFP-Puro) Expression Lentivirus, ConcentratedLVP679-PBS$650.00
Cas9 (CMV, Zeo) lentiviral particles, in vivo readyLVP1391-PBS$650.00
Cas9 (EF1a, Bsd) Expression Lentivirus, ConcentratedLVP685-PBS$650.00
Cas9 (EF1a, GFP-Bsd) Expression Lentivirus, ConcentratedLVP710-PBS$650.00
Cas9 (EF1a, GFP-Puro) Expression Lentivirus, ConcentratedLVP708-PBS$650.00
Cas9 (EF1a, Neo) Expression Lentivirus, ConcentratedLVP686-PBS$650.00
Cas9 (EF1a, No Selection) lentiviral particles, in vivo readyLVP1394-PBS$650.00
Cas9 (EF1a, Puro) Expression Lentivirus, ConcentratedLVP684-PBS$650.00
Cas9 (EF1a, RFP-Bsd) Expression Lentivirus, ConcentratedLVP711-PBS$650.00
Cas9 (EF1a, RFP-Puro) Expression Lentivirus, ConcentratedLVP709-PBS$650.00
Cas9 (EF1a, Zeo) lentiviral particles, in vivo readyLVP1392-PBS$650.00
CRISPR / h Cas9 Adenovirus, in vivo readyAVP010-PBS$650.00
CRISPR / hCas9 expression AdenovirusAVP010$395.00

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  • Home
  • Products
    • Premade Lentivirus
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    • Cell-Specific Reporter
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    • Non-integrating LV
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    • shRNA lentivirus
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    • Control lentivirus
    • Ultra titer lentivirus
    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
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    • Protein Expression
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