Cas9 Expression
About CRISPR-Ca9:
Cas9 (CRISPR associated protein 9) is endonuclease that can cut the double-strand DNA, and thereby alters a cell’s genome. CRIAPS-Ca9 genomic gene-editing technology utilizes a guide RNA molecule (gRNA) to direct a Cas endonuclease to the genomic DNA targeted site. Then, the Cas enzyme creates a double-strand break that can be repaired either by Non-Homologous End-Joining (NHEJ), which can result in insertion or deletions (InDels), or by Homology Directed Repair (HDR) when a repair Donor DNA template is present.
InDels can disrupt the expression of the target gene (So-called, KnockOut). While repaired by HDR, the genome was modified with a foreign gene (So-called, KnockIn), or with sequence corrected gene (targeted gene therapy or gene repair).
Cas9 (from Streptococcus pyogenes ) is the most frequently used cas endonuclease in CRISPR. Ca9 protein can be delivered as the standalone expression, or delivered with a target gRNA in a one-vector system.
GenTarget offers standalone Cas9 Expression Lentivirus, and other modified Cas9 enzymes. To avoid the unwanted permanent Cas9 expression, Gentarget also provides the Ca9 Expression Adenovirus, and the Integration-Deficient Lentivirus for Cas9 expression.
Cas9 Expression Products:
Our Cas9 lentivirus are produced from our proprietary high-titer lentivectors that express a nuclear penetrating humanized wild-type Cas9 endonuclease (Streptococcus pyogenes). The Cas9 enzyme is driven by different promoters with a variety of antibiotic selection markers (see the core expression lentivector scheme below), providing you an easy delivery for cas9 expression in almost all cell types, including the hard-to-transfected cell types, primary cells, and non-dividing cells, which makes the gene-editing possible in all cell types.
Cas9 Lentivirus Product Features:
- High Cas9 expression Level by enhanced promoters to fit different cell types;
- High titer lentivirus provides more efficient Cas9 delivery in almost all cell types including primary cells and non-dividing cells;
- Flexible selection with different antibiotic and fluorescent markers;
- Best nuclear penetrating with enhanced NLS leader: the Cas9 is expressed with an optimized, proprietary Nuclear Localization Signal (NLS), providing the efficient cas9 delivery into the nuclear region where the gene-editing occurs.
- Easy to use: you can simply synthesize the gRNA (or use gRNA lentivirus) and used it together with the Cas9 lentivirus for targeted gene editing.
- The standalone Cas9 lentivirus allows generating CRISPR-ready animal models or stable cell lines in your desired cell types or animals that are used as a model for any gene editing.
Please see Cas9 Lentivirus Product Manual, or see each product detail below.