rtTA Expression

rtTA (reverse tetraccycline Transcriptional Activator) Lentiviral Particles 

These Pre-made lentiviral particles express the reverse tetraccycline transcription activator gene rtTA-M2 (ABC65845.1) under an enhanced EF1α promoter and express an selection marker (Neo, Puro, Bsd or a fluorescent-antibiotic fusion dual marker) under a constitutive Rsv promoter.  rtTA-M2 is a mutant of rtTA that has increased stability and reduced background expression in the absence of the tetra_cycline or Dox and improved inducibility in the presence of Tet / Dox. 

Please see the core map scheme below for details.

 rtTA map scheme

 Applications: rtTA binds to the tetra_cycline operator element (TetO) in the presence of doxyscycline (Dox). Any promoters embedded with TetO sequences can bind to the rtTA protein when the inducer (Dox or tetraccycline) is present, activating transcription of the downstream gene (the so-called Tet-On inducible system).   

See Product Manual (.pdf) here.  

Note: please see our “Pre-made rtTA Expression Stable Cell Lines” to generate your own inducible target expression cell lines.

Reference:

  1. PNAS, July 5, 2000, V97(14), 7963-7968
  2. BMC Developmental Biology 2010, 10:17

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