Transformed from huamn lung cancer cell line, A549 by lentiviral system with firefly luciferase gene II and GFP. The Luciferase and GFP was co-expressed under CMV promoter as seperated protein (not as fusion). The Puromycin marker was expressed under Rsv promoter. The cell constitutively co-expresses both firefly Luciferase and GFP. The following express cassette was integrated into cell line’s genome.
Each cell demonstrates strong GFP fluorescent signal and luciferase assay signal (Note: this is pooled stable ell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells).
Please see details in Product Manual.
Sold at: 1 vial x (2 x 106 cells)/vial