Recombinant Adeno-Associated Virus (rAAV)

Adeno-Associated Virus (AAV) is a widely used viral vector system for delivering genetic material into various cell types, both in vitro and in vivo. AAV is a small (~25nm), non-enveloped virus belonging to the Parvovirus family. 

The recombinant form of AAV (rAAV) has been engineered to remove all viral genes, leaving only the necessary inverted terminal repeats (ITRs) to enable packaging and genome stability. The Helper-free AAV system use three plasmids: transgene, pAAV-RC and Helper, to package the rAAV. The transgene plasmid does not share any regions of homology with the rep/cap-gene containing plasmid (pAAV-RC), preventing the production of wild-type AAV through recombination. This modification makes rAAV a safe and efficient tool for gene delivery in a wide range of research and therapeutic applications. Click AAV FAQ for more details.

Why use rAAV?

AAV has several advantages over other viral vectors, making it a preferred choice for many applications:

• Safety: Unlike Lentivirus, AAV does not integrate into the host genome, reducing the risk of insertional mutagenesis and making it ideal for long-term yet non-disruptive gene expression.
• Long-Term Expression: AAV can persist in cells as episomal DNA, enabling sustained gene expression without genome integration.
• Broad Tropism: Different AAV serotypes can target a variety of tissues, including liver, muscle, brain, and retina, allowing for flexibility in research and therapeutic applications.
• Low Immunogenicity: Compared to Adenovirus, AAV elicits a much lower immune response, making it a better option for in vivo applications, including gene therapy.
• Efficient Transduction: AAV efficiently transduces dividing and non-dividing cells, providing versatility across different experimental and clinical settings.

About Gentarget Inc’s rAAV.

Gentarget’s premade Recombinant Adeno-Associated Virus (rAAV) are packaged in HEK293T cells using three plasmids: the transgene plasmid, the helper-plasmid and the Rep/Cap plasmid. see AAV package scheme below:

All pre-made rAAV are in stock, ready to ship, provided as 100ul aliquots at the concentrated titer of 1×10^12 GC (genomic copy) /ml. Gentarget Provides 11 Serotypes as: AAV1 to AAV10, AAV-DJ, for categories like AAV-Fluorescent Reporters (see transduction sample image below), AAV for CARs, TCRs, CRISPR-Cas9, Cell immortalization, and much more.

click Product Manual for product details.

Customized AAV virus

GenTarget also Provides customized AAV services, from cloning to packaging for your desired target with your desired serotypes. Please Contact us with your request details for a service quote!