Cancer Specific Report
About Survivin Promoter:
Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5 a member of the inhibitor of apoptosis family. It is expressed highly in most human tumors and fetal tissue, but is completely absent in normal adult tissues. Survivin promoter is a cancer-specific promoter for various cancers. Thus, Survivin promoter is used to induce cancer-specific transgene expression. Survivin expression is up-regulated at the transcriptional level in tumor tissues via different signal pathway, such as hypoxia.
Specifically Label Cancer Cells:
GenTarget developed a set of reporting lentivirus for selectively labeling most common cancer cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human Survivin gene (click to see its sequence) that over-expressed in most common human cancers. Those reporter lentivirus are best suitable for specifically labeling various cancer cells, as well as for the signal pathway research on Survivin promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
see Product Manual for details (.pdf).