CRE and Flp reporting cells

 1. About LoxP cassette for CRE based color-switch:

Those reporting cell lines, derived from HEK293 cells, constitutively expresses a “LoxP-GFP-Stop-LoxP-RFP-Stop” cassette or “LoxP-mCherry-Stop-LoxP-GFP-Stop“, under CMV promoter. See the genomic integrated express cassette structure below.

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When CRE enzyme is present, CRE-LoxP recombination will excise out the front fluorescent reporter between two loxP sites, resulted in the expression of the back-fluorescent reporter  (turned on). 

 2. About FRT cassette  for Flp based color-switch:

Those reporting cell lings express the “FRT-GFP-stop-FRT-RFP-Stop” cassette under either an enhanced CMV promoter or EF1a promoter. See the genomic integrated express cassette structure below.

When FLP protein is present in the nucleus, the FLP excises / deletes the DNA fragment between two FRT sites.  As a result, the GFP is removed and RFP is expressed, resulted in high RFP expression.

see details in CRE Reporting cell lines Manual  or Flp /FRT Reporting Cell line Manual.

Product Citations:

1. ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrp rd6 mouse;
2. In vivo screening identifies GATAD2B as a metastasis driver in KRAS-driven lung cancer;