This A549 / Cas9 Stable Cell Line is transformed from the A549 human lung cancer cell line, stably expressing the nuclear penetrating humanized wild-type Cas9 endonuclease (Streptococcus pyogenes). The Cas9 enzyme is driven by our engineered super strong CMV promoter (suCMV). It also contains the RFP-Puromycin fluorescent-antibiotic fusion dual marker under the RSV promoter. Each cell expresses high level of Cas9 enzyme and also demonstrates the RFP fluorescent signal under fluorescent-microscope. see the expression cassette below that integrated into cell line’s genome.
This CRISPR ready (Cas9 expression) stable cell lines make the genomic editing easier than ever in A549 cells. You simply deliver the target specific gRNA into the CRISPR ready cell line and select the knock-out or knock-in cells for your desired target. No need to do cas9 containing CRISPR cloning, and no worry about the hard-to-delivered Cas9 constructs. You only need to generate the much easier in cloning, and much smaller in size of the target-gRNA constructs. GenTarget also provide services to generate your target specific, ready-to-use gRNA lentivirus. Please contact us if you need the CRISPR gRNA lentivirus services.
Sold at: 1 vial x (2 x 106 cells)/vial, Product Manual.