Description
Pre-packaged rAAV in Serotype-DJ, constitutively express an Anti-CD19 CAR (Anti-CD19-ScFv-4D28-CD3ζ) with N-terminal 3X FLAG (DDK) tag, under a enhanced CMV Promoter (which is tested strong in T cells and B cells).
The ScFv of anti-CD19 (Click to see its sequence) (the variable region sequences of the FMC63 monoclonal antibody which recognizes human CD19), is fusion with the CAR (Chimeric Antigen Receptor) of “CD8 hinge, CD28 co-stimulatory domain, and CD3ζ signaling domains”. Its expression can be detected by anti-FLAG (DDK tag) antibody. The following scheme showed the AAV vector’s core structure.
CD19 is a marker of in most B cell leukemias and lymphomas but not in any normal tissue other than the B cell lineage, CD19 is used to diagnose cancers that arise from this type of cell, and is used as the target for CD19-targeted therapies. The AAV product is used as CAR-T targeted killing of the CD19 positive cancer cells. See Product Manual for details.
Significant strides have been made in T cell therapies targeting well-characterized tumor antigens, including CD19 / CD20, CD22, Her2, BCMA HLA-A2, TGFβ specific-CAR. Please see also Gentartet’s CAR-T Lentivirus product, click CAR-T Lentivirus Manual, and click Anti-CD19 CAR-T Manual to see all products. Please also look at our TCR Lentivirus page. Note: all products are for research use ONLY.
AAV Serotype-DJ is a synthetic serotype made from different serotypes using DNA shuffling. AAV-DJ serotype has the enhanced transduction efficiency in vitro and in vivo compared to wild-type serotypes with Broad Cell Tropism, great transduction rate in CNS, Liver, Kidney, Fibroblasts, muscle.
Titer: ~ 1×10^12 GC/mL
Size: 100ul/vial, in stock, ready to ship in overnight Dry-ice package.
Storage Buffer: PBS
CAT#: A36.DJ
