Description
Pre-packaged rAAV in Serotype-DJ, constitutively express an Anti-CD19-Anti-CD20 CAR [(CD19-scFv)-(G4S)x4-(CD20-scFv)-41BB-CD3ζ] with N-terminal 3X FLAG (DDK) tag, under a enhanced CMV Promoter (which is tested strong in T cells and B cells).
The Bispecific Anti-CD19/CD20 (Click to see its sequence), is fusion with the CAR (Chimeric Antigen Receptor) of “CD8 hinge, 4-1BB co-stimulatory domain, and CD3ζ signaling domains”. Its expression can be detected by anti-FLAG (DDK tag) antibody and anti G4S (GGGGS) antibody . The following scheme showed the AAV vector’s core structure.
CD19 is a marker of in most B cell leukemias and lymphomas but not in any normal tissue other than the B cell lineage. CD20 is another B cell surface protein involved in B cell activation and differentiation. Both CD19 and CD20 is used to diagnose cancers that arise from this type of cell, and used as the target for CD19/CD20-targeted CAR-T therapies (Reference). See Product Manual for details.
Significant strides have been made in T cell therapies targeting well-characterized tumor antigens, including CD19 / CD20, CD22, Her2, BCMA HLA-A2, TGFβ specific-CAR. Please see also Gentartet’s CAR-T Lentivirus product, click CAR-T Lentivirus Manual, and click Anti-CD19 CAR-T Manual to see all products. Please also look at our TCR Lentivirus page. Note: all products are for research use ONLY.
AAV Serotype-DJ is a synthetic serotype made from different serotypes using DNA shuffling. AAV-DJ serotype has the enhanced transduction efficiency in vitro and in vivo compared to wild-type serotypes with Broad Cell Tropism, great transduction rate in CNS, Liver, Kidney, Fibroblasts, muscle.
Titer: ~ 1×10^12 GC/mL
Size: 100ul/vial, in stock, ready to ship in overnight Dry-ice package.
Storage Buffer: PBS
CAT#: A37.DJ
