Description
Pre-packaged rAAV in Serotype-DJ, constitutively express MART-1 specific T Cell Receptor (TCR) (Clone DMF5), under a enhanced CMV Promoter (which is tested strong in T cells and B cells). See Product Manual for details.
This TCR construct (Click to see its sequence) specifically recognizes a Peptide (peptide 1: MMWDRGLGMM) in antigen MART-1 (Melanoma-associated antigen recognized by T cells-1, or Melan-A). The TCR α chain variable region with a transmembrane (TM) domain is linked with TCR β chain variable region with a transmembrane (TM) domain, by F2A element, as “TCRα(V)/Tm-F2A-TCRβ(V)/Tm”. The following scheme showed the AAV vector’s core structure.
MART-1, also known as Melan-A, is a protein antigen found on the surface of melanocytes. The MART-1 antigen is a target in immunotherapy for patients with melanoma. TCRs targeting MART-1 recognize the antigen on melanoma cells, leading to T cell activation and cytotoxic T cell response to malignant melanoma cells. References: Ref 1, Ref 2.
Significant strides have been made in T cell therapies targeting well-characterized tumor antigens, including CD19 / CD20, CD22, Her2, BCMA HLA-A2, TGFβ specific-CAR. Please see also Gentartet’s CAR-T Lentivirus product, click CAR-T Lentivirus Manual, and click Anti-CD19 CAR-T Manual to see all products. Please also look at our TCR Lentivirus page. Note: all products are for research use ONLY.
AAV Serotype-DJ is a synthetic serotype made from different serotypes using DNA shuffling. AAV-DJ serotype has the enhanced transduction efficiency in vitro and in vivo compared to wild-type serotypes with Broad Cell Tropism, great transduction rate in CNS, Liver, Kidney, Fibroblasts, muscle.
Titer: ~ 1×10^12 GC/mL
Size: 100ul/vial, in stock, ready to ship in overnight Dry-ice package.
Storage Buffer: PBS
CAT#: A49.DJ
