This Pre-made lentivirus expresses human target, AKR1C3 (human aldo-keto reductase family 1 member C3) (alternative name: DD3; DDX; HA1753; HAKRB; HAKRe; hluPGFS; HSD17B5; PGFS). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_003739.5
The full sequence-verified target is natively expressed under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without needs for any induction) or optionally used for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.
A GFP-Puromycin fusion dual marker under the RSV promoter allows fluorescence sorting or Puromycin resistance selection of transduced cells. The GFP signal additionally provides convenient, real-time monitoring of the particles’ performance.
Each Lentivirus is validated on lot-by-lot basis, and target expression is guaranteed. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).