Lentivirus expresses codon optimized Blue Fluorescent Protein (BFP) under suCMV promoter. A Blasticidin marker under Rsv promoter allows to select transduced fluorescent positive cells. See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create BFP expression stable cell line (via Blasticidin selection);
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.