Pre-made BFP (Blue Fluorescent Protein) lentivirus expressing codon optimized BFP under EF1a promoter. A Puromycin (Puro) gene under Rsv promoter allows to select transduced fluorescent positive cells.
The particles was concentrated and buffer exchanged into PBS. (See product manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create BFP expression stable cell line (via Blasticidin selection);
Amount: 1 x 108 IFU/ml x 200ul in PBS.