Pre-made BFP (Blue Fluorescent Protein) lentivial particles expressing codon optimized BFP under EF1a promoter. A Puromycin (Puro) gene under Rsv promoter allows to select transduced fluorescent positive cells. See product manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create BFP expression stable cell line (via Blasticidin selection);
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.