Lentivirus express human codon-optimized Cas9 endonuclease (Streptococcus pyogenes ) under CAG promoter, with a proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. CAG promoter has strong promoter strength in some cell types where the CMV, or EF1a promoter strength is weak.
It also contains a Blasticidin antibiotic marker for the selection of the transduced cells.
See Product Manual here (.pdf).
Application: used for CRISPR gene editing ( Note: used with a separate “gene-specific gRNA lentivirus” or ” gene-specific gRNA vector” for CRISPR editing )
Amount: 5 x 106 IFU/ml x 200ul in DMEM medium with 10% FBS, 10x of polybrene.
Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.