Description
Lentivirus express human codon-optimized Cas9 endonuclease (Streptococcus pyogenes ) under CAG promoter, with a proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. CAG promoter has strong promoter strength in some cell types where the CMV, or EF1a promoter strength is weak.
It also contains a Neomycin (G418) antibiotic marker for the selection of the transduced cells.
This is the concentrated lentivirus with higher titer. See Product Manual here (.pdf).
Application: used for CRISPR gene editing
Amount: 1×10^8 IFU/mlx 200ul (provided in PBS solution, premixed with 10x Polybrene, 60ug/ml)
Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.
Cat#: LVP689-PBS