Pre-made firefly Luciferase (luc) Lentiviral particles expressing the firefly luciferase gene under the CAG promoter. A GFP–Puromycin fusion dual marker was expressed under a different RSV promoter, which allows to sort or select transduced cells for long term expression via GFP signal or via Puromycin antibiotic (Dual selection). GFP signal provides a convenient, realtime monitoring the particles performance. See Product Manual for details (.pdf).
Particles was concentrated and buffer exchanged into PBS as in vivo ready status.
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.