Pre-made firefly Luciferase (luc) Lentiviral particles expressing luciferase II gene under the CAG promoter. A RFP–Puromycin fusion dual marker was expressed under a different RSV promoter, which allows to sort or select transduced cells for long term expression via RFP signal or via Puromycin antibiotic (Dual selection). RFP signal provides a convenient, realtime monitoring the particles performance. See product manual for details (.pdf).
Particles was concentrated and buffer exchanged into PBS as in vivo ready status.
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.