Cas9 (CMV, RFP-Bsd) Integrase-Deficient Lentivirus

$395.00

SKU: IDLV010A Category:

Description

Non-Integrating Lentivirus express human codon-optimized wild-type Cas9 endonuclease (from Streptococcus Pyogenes, click to see its codon sequence) under suCMV promoterwith our proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. It also contains the RFPBlasticidin Fluorescent-antibiotic fusion dual marker that used for transient selection (within 1-2 weeks post-transduction), when desired, via antibiotic killing or Fluorescent sorting. (See also the related regular lentivirus, CAT#: LVP707).

See Product Manual here (.pdf).

Application: transient delivery Cas9 protein for CRISPR editing, without the unwanted insertional mutagenesis

( Note: used with the “gene-specific gRNA lentivirus” that needed and not included,  for CRISPR gene editing )

Amount:  5 x 106 IFU/ml x 200ul in PBS, pre-mixed with polybrene (10x, 60 ug/ml). 

Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.

Cat#: IDLV010A