Cas9 (EF1a, GFP-Bsd) Integrase-Deficient Lentivirus

$395.00

SKU: IDLV009B Category:

Description

Non-Integrating Lentivirus express human codon-optimized wild-type Cas9 endonuclease (from Streptococcus Pyogenes, click to see its codon sequence) under the enhanced EF1a promoter, with our proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. It also contains a GFP-Blasticidin Fluorescent-antibiotic dual marker that used for transient selection (within 1-2 weeks post-transduction), when desired, via antibiotic killing or fluorescent sorting. (See also the related regular lentivirus, CAT#: LVP710.

See Product Manual here (.pdf).

Application: transient delivery Cas9 protein for CRISPR editing, without the unwanted insertional mutagenesis

( Note: used with the “gene-specific gRNA lentivirus” that needed and not included,  for CRISPR gene editing )

Amount:  5 x 106 IFU/ml x 200ul in PBS, pre-mixed with polybrene (10x, 60 ug/ml). 

Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.

Cat#: IDLV009B