Premade Lentivirus (in stock, ready to ship), expresses human target, CD22 (human CD22 molecule) (alternative name: SIGLEC-2; SIGLEC2). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID:NM_001278417.1 .
The target is expressed with C-terminal 6His tag under an optional inducible suCMV promoter which means this lentivirus can be used for regular constitutive high expression (without any induction), or used for inducible expression by tetracycline or Dox induction when its repressor protein (TetR) is present. The TetR can be purchased separately if desired for inducible expression.
It contains the constitutively expressed Blasticidin–RFP fusion dual marker under the RSV promoter, which allows to select the transduced cells via fluorescence sorting or blasticidin antibiotic killing. The RFP signal additionally provides convenient, real-time monitoring of the particles’ performance. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium (premix with Polybrene). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).