h CD28 (6His) inducible lentiviral particles

$395.00

SKU: LVP1078 Categories: ,

Description

Pre-made Lentivirus expresses human target, CD28 (human CD28 molecule) (alternative name: TP44). The sub-cloned codon sequence is identical (100% match) to CDS region  in NCBI ID:NM_006139.3.

The full sequence-verified target is expressed with the C-terminal 6His tag under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.

BlasticidinRFP fusion dual marker under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of  transduced cells.  The RFP signal additionally provides convenient, real-time monitoring of the particles’ performance.  See Product Manual for details (.pdf).

Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium, pre-mixed with polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).

Cat#: LVP1078