Lentivirus expresses mouse target: CD40LG (musculus CD40 ligand), (alternative name: CD154; CD40-L; Cd40l; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP), with coding sequence 100% matching to CDS region in NCBI ID: NM_011616. It contains a RFP–Blasticidin dual selection marker.
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). It constitutively expresses the target, but also optional can be used as for the inducible expression when used together with its repressor (TetR). A RFP-Blasticidin fusion dual selection marker under RSV promoter, allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic. RFP signal provides a convenient, realtime monitoring the particles performance. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).