Pre-made Lentivirus expressing human cyclin dependent kinase 4, CDK4 gene (NM_000075.), under enhanced EF1a promoter. It is used to immortalize human bronchial cells and myogenic cells. A GFP–Puromycin Fusion dual marker was expressed under RSV promoter, which allows to select the transduced cell via Puromycin antibiotic or via cell sorting by GFP fluorescent signal.
It is used for cell immortalization and other application. See Product Manual (.pdf).
Amount: This is the concentrated lentivirus, provided in PBS solution as 200ul/per vial at 1 x 108 IFU/ml, best used for serum free cell culture, hard-to-infected cell types, or In Vivo application.