Pre-made CFP lentivial particles containing codon optimized cyan fluorescent protein (CFP) under suCMV promoter. A Blasticidin antibiotic selection under Rsv promoter allows to select transduced fluorescent positive cells. The following scheme showed the lentivector core structure:
This lentivirus was concentrated and buffer changed into PBS, best suitable for difficult-to-tansduced cell types, or for in vivo application.
- monitor lentivirus transduction efficiency for your cells of interest;
- create CFP expression stable cell line (via Blasticidin selection);
Amount: 1 x 108 IFU/ml x 200ul in PBS