Pre-made CFP lentivial particles expressing codon optimized CFP under engineered EF1a promoter. It contains a Puromycin antibiotic selection marker under Rsv promoter.
The re-engineered EF1a promoter is strong constitutive promoter. It is non-tissue specific, highly expressed in all cell types, and less likely be silenced after long-term culture. See Product manual for details (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- stable cell line generation;
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.