Lentivirus express a fusion target of (CFP-human Histone 2B). The human Histone 2B (H2B) codon sequence was fusioned, at its N-terminal, with CFP (Cyan Fluorescent Protein), expressed under a enhanced suCMV promoter, containing a Puromycin antibiotic marker under the RSV promoter.
Fluorescent labeled H2B allowed high-resolution imaging of both mitotic chromosomes and interphase chromatin, and the latter revealed various chromatin condensation states in live cells. It can directly observe the Double Minute Chromosomes (DMs) in living cells without compromising nuclear and chromosomal structures.
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Amount: 200ul/vial (1×107 IFU/ml), provided in DMEM, containing 60ug/ml of Polybrene (10x).