Pre-made CFP lentivial particles expressing codon optimized CFP under suCMV promoter. A Neomycin gene under RSV promoter allows to select transduced fluorescent positive cells. See product manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create CFP expression stable cell line (via Neomycin selection);
Amount: 1 x 107 IFU/ml x 200ul in in DMEM medium with 10% FBS and 60ug/ml of polybrene.