Pre-made lentiviral particles expressing a fusion target of (CFP-human Zyxin). The sequence verified codon sequence of human Zyxin gene was fusioned at its N-terminal with CFP protein, expressed under a consitutive suCMV promoter, containing a puromycin antibiotic marker under the RSV promoter.
Human Zyxin gene (NCBI accession ID: NM_001010972.1) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. The fluorescent labeled Zyxin can be used to visualize sub-cellular singal transduction pathway where the adhesion-stimulated gene expression and the cytoskeletal organization of actin bundles happened.
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Amount: 200ul/vial (1x10e7 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x).