Luciferase (firefly)-2A-GFP (CMV, Puro), concentrated Lentivirus



Pre-made Lentivirus express firefly  luciferase 3 gene under a modified  CMV promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically and constitutively expressed as two individual proteins,  mediated by a 2A element.  

Puromycin antibiotic marker was expressed under RSV promoter, allows to sort or select transduced cells for long-term expression via GFP signal or via Puromycin antibiotic (Dual selection).  GFP signal provides convenient, real-time monitoring performance of lentivirus in your cell type.  See Product Manual for details (.pdf).

This is the concentrated lentivirus, and buffer exchanged into PBS solution.

Amount:  200ul/per vial, at 1 x 108 IFU/ml in PBS. 

Cat#: LVP020-PBS