Pre-made lentiviral particles bicistronically express two individual proteins: nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. It does not contain any antibiotic selection marker. This particles was concentrated and buffer changed into PBS solution, best suitable for hard-to-transduced cell lines, serum sensitive cell lines and in vivo applications.
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. CRE lentiviral particles can catalyze the jointing event In Vivo in the presence of lox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.
Particles was concentrated and resuspended in PBS solution, best suitable for hard-to-transduced cell line, or serum-free cell culture, or directly used for in vivo application.
See Product manual for details (.pdf).
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.