Pre-made lentiviral particles bicistronically express two individual proteins: nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. CRE contains a NLS (nuclear localization signal) at its N-term. This nuclear permeant CRE increases the recombination events in vivo. A Puromycin marker was expressed under RSV promoter. Particles allows to sort or select transduced cells for long term expression via GFP signal or via Puromycin antibiotic selection.
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. CRE lentiviral particles can catalyze the jointing event In Vivo in the presence of lox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.
NLS-CRE and GFP are constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expression as desired. GFP signal provides a convenient, realtime monitoring the particles performance. See Product manual for details (.pdf).
Amount: 200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS.