Pre-made lentiviral particles bicistronically express two individual proteins: nuclear permeable CRE recombinase and the GFP marker under the same EF1a promoter. A Puromycin marker was expressed under RSV promoter. Particles allows to sort or select transduced cells for long term expression via GFP signal or via Puromycin antibiotic selection.
CRE and GFP are constitutively expressed in high level, indenpendent of cell types. GFP signal provides a convenient, realtime monitoring the particles performance.
CRE protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. CRE lentiviral particles can catalyze the jointing event In Vivo in the presence of lox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.
Particles was concentrated and resuspended in PBS solution, best suitable for hard-to-transduced cell line, or serum-free cell culture, or directly used for in vivo application.
See Product Manual for details (.pdf).
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.