Pre-made lentivirus express CRE recombinase under inducible suCMV promoter. CRE contains a NLS (nuclear localization signal) at its N-term. This nuclear permeant CRE increases the recombination events in vivo. A Puromycin marker under RSV promoter, allows to select transduced cells for long term expression via blasticidin antibiotic.
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. CRE lentiviral particles can catalyze the jointing event In Vivo in the presence of lox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.
Particles was concentrated and resuspended in PBS solution, best suitable for hard-to-transduced cell line, or serum-sensitive cell lines and for in vivo application.
NLS-CRE is constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expression as desired. See Product Manual for details (.pdf)
Amount: 200ul/per vial, at 1 x 108 IFU/ml in PBS.