Pre-made lentivirus express firefly Renilla Luciferase reporter under a minimal promoter inserted with 8 tandem repeats of CRE_TRE (cAMP Response Element_Transcription Response Element), for detecting or monitoring cAMP-PKA signaling pathway acitvity.
This lentivirus also contain the puromycin selection marker under the constitutive RSV promoter for antibiotic selection. See Product Manual for details (.pdf).
If desired, you can also use a pathway “Control Lentivirus” to establish the No-response control profile to your pathway stimulus. The corresponding pathway control lentivirus is: CAT#: Path-Ctr4.
This is concentrated lentivirus provided in PBS solution, used for hard-to-transduced cell types or for serum-free cell culture.
Amount: 200ul/per vial, at 1 x 108 IFU/ml in PBS.